Dynamics of PI(4,5)P_2-rich vesicles in PC12 cells.
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چکیده
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Vesicular catecholamine release has been measured amperometrically from undifferentiated rat PC12 cells using carbon fiber microelectrodes. During superfusion with high K(+) saline, vesicular release was detected from approximately 50% of 200 cells investigated. On repeated stimulation the releasable pool of vesicles is rapidly depleted, while vesicle contents remains constant. Vesicular catech...
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Two distinct population of vesicles can be identified in PC12 cells by subcellular fractionation and immunofluorescence. Density gradient separation reveals one population of dense vesicles that contains the transgenic regulated secretory protein hGH (human growth hormone) along with the endogenous neurotransmitter norepinephrine. Some of the neuronal vesicle marker synaptophysin (P38) is also ...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2003
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.43.s164_4